منابع مشابه
Holding the reins on myosin V.
M yosins comprise a diverse family of molecular motor enzymes that use the energy from cycles of ATP binding, hydrolysis, and product release to perform mechanical work along actin filaments. Although all characterized myosins share a conserved catalytic motor domain, referred to as ‘‘head,’’ variations in enzymatic and structural properties allow different myosins to generate diverse types of ...
متن کاملMyosin reins in neurites
Myosin reins in neurites yosin II pulls growth cones in the right direction, as shown by Stephen Turney and Paul Bridgman (Washington University, St. Louis, MO). Growing neurons in the developing embryo are directed by guidance cues such as laminin-1 (LN1), which steer the extension of neurite growth cones. Bridgman had previously noticed that neuronal growth cones contain high levels of myosin...
متن کاملMyosin V
Calcium activates the ATPase activity of tissue-purified myosin V, but not that of shorter expressed constructs. Here, we resolve this discrepancy by comparing an expressed full-length myosin V (dFull) to three shorter constructs. Only dFull has low ATPase activity in EGTA, and significantly higher activity in calcium. Based on hydrodynamic data and electron microscopic images, the inhibited st...
متن کاملMyosin V motor proteins
Mammalian myosin V motors transport cargo processively along actin filaments. Recent biophysical and structural studies have led to a detailed understanding of the mechanism of myosin V, making it perhaps the best understood cytoskeletal motor. In addition to describing the mechanism, this review will illustrate how "dynamic" single molecule measurements can synergize with "static" protein stru...
متن کاملMyosin V stepping mechanism.
We observe the myosin V stepping mechanism by traveling wave tracking. This technique, associated with optical tweezers, allows one to follow a scattering particle in a two-dimensional plane, with nanometer accuracy and a temporal resolution in the microsecond range. We have observed that, at the millisecond time scale, the myosin V combines longitudinal and vertical motions during the step. Be...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2005
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0507068102